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沃特世蛋白質反相色譜柱 介紹：
蛋白質反相色譜柱
蛋白質反相色譜分析是一種基于蛋白質在溶液中的相對疏水性的分離技術，分析時使用裝填有短鏈鍵合相的大孔徑顆粒（例如，300Å）的色譜柱。如果使用離子對試劑（例如0.1% TFA）來zui大限度減少不良離子干擾，通常利用逐漸升高的有機溶劑濃度梯度來控制分離。一般來說，洗脫順序取決于蛋白質或蛋白質亞基的疏水性，疏水性zui弱的蛋白質將首先洗脫出來。此外，顆粒組成（硅膠vs雜化顆粒）、孔徑、鍵合相類型和密度，以及分離條件（例如梯度持續(xù)時間、分離溫度、流速）等等對于實現(xiàn)滿足應用要求的分離而言也非常重要。
沃特世蛋白質反相色譜柱部分色譜柱：
 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 100 mm, 1/pkg 部件代碼: 186007568 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 50 mm, 1/pkg 部件代碼: 186007567 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pkg 部件代碼: 186007569 | ---- |
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| DeltaPak C4 Guard Column, 300Å, 5 µm, 3.9 mm X 20 mm, 2/pkg 部件代碼: WAT046885 | ---- | ||
| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pkg 部件代碼: 186007495 | ---- |
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| DeltaPak C4 Column, 300Å, 15 µm, 3.9 mm X 300 mm, 1/pkg 部件代碼: WAT011812 | ---- | ||
| DeltaPak C4 Guard-Pak Insert, 300Å, 5 µm, 10/pkg 部件代碼: WAT036865 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pkg 部件代碼: 186007493 | ---- |
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| ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pkg 部件代碼: 186007494 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 50 mm, 1/pkg 部件代碼: 186005589 | ---- |
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| ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg 部件代碼: 186004899 | ---- |
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| ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg 部件代碼: 186006549 | ---- | ||
| BioSuite Phenyl Column, 1000Å, 10 µm, 2 mm X 75 mm, 1/pkg 部件代碼: 186002156 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 100 mm, 1/pkg 部件代碼: 186005590 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 150 mm, 1/pkg 部件代碼: 186005591 | ---- |
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| DeltaPak C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg 部件代碼: WAT011794 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg 部件代碼: 186004497 | ---- |
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| ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg 部件代碼: 186004623 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg 部件代碼: 186004496 | ---- |
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| DeltaPak C4 Column, 100Å, 5 µm, 3.9 mm X 150 mm, 1/pkg 部件代碼: WAT011796 | ---- |
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| ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg 部件代碼: 186004495 | ---- |
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| DeltaPak C4 Column, 100Å, 15 µm, 3.9 mm X 300 mm, 1/pkg 部件代碼: WAT011807 | ---- |
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